anti il 6 receptor antibody  (R&D Systems)

Journal: iScience

Article Title: Evolution of tumor stress response during cytoreductive surgery for ovarian cancer

doi: 10.1016/j.isci.2025.112317

Figure Lengend Snippet: IL-6 receptor blockade partially restores tumor control in the setting of surgical intervention (A) Pre- and postoperative plasma IL-6 cytokine concentration (pg/μL) for mice with ovarian cancers undergoing laparotomy or not. We used the Mann-Whitney test to compare plasma IL-6 concentration of mice undergoing laparotomy or not. (B) Experimental design. (C) Representative experiment: graphical representation of bioluminescence by luciferin uptake in ovarian cancer tumors in mice undergoing laparotomy treated with either control IgG or anti-IL6R antibody, by total flux (p/s). We also compared bioluminescence by luciferin uptake in ovarian cancer tumors in mice not undergoing laparotomy treated with either control IgG or anti-IL6R antibody, by total flux (p/s). We used Mann-Whitney U test to compare mean area under the curve between the two groups. Data are represented as mean with standard error of mean. ∗ p < 0.05. IL, interleukin; IL-6R, interleukin-6 receptor; ns, not significant.

Article Snippet: Mice received intraperitoneal injection of either 400 μg of anti-IL-6 receptor (IL6R) antibody ( InVivo MAb anti-mouse IL6R, clone 15A7, BioxCell) or 400 μg of control IgG ( InVivo MAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin, clone LTF-2, BioxCell) 24 h prior to laparotomy/no laparotomy, 24 h post-intervention, and then weekly for 2 weeks.

Techniques: Control, Clinical Proteomics, Concentration Assay, MANN-WHITNEY